Introduction To Science

👤 WHO SHOULD ATTEND

◆ Experienced chromatographers with a good working knowledge of HPLC
◆ Analysts involved in HPLC method development and optimisation
◆ Laboratory professionals responsible for method validation, transfer or troubleshooting

📋 PREREQUISITES

✓ Solid grounding in chromatography and chemistry
✓ Practical HPLC user experience
✓ Familiarity with system suitability parameters
✓ Previous method-development experience is recommended
✓ Completion of Understanding HPLC and Troubleshooting is advantageous

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🎯 LEARNING OUTCOMES

By the end of this course, you will be able to:

✓ Define clear method-development objectives aligned with sample and regulatory requirements

✓ Optimise sample preparation, column chemistry and mobile phase conditions for improved selectivity and resolution

✓ Apply Quality by Design (QbD) principles to build robustness into analytical methods

✓ Select appropriate columns using the Hydrophobic Subtraction Model (HSM)

✓ Develop and optimise isocratic and gradient HPLC separations

✓ Evaluate system suitability, robustness, repeatability and pre-validation requirements

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KEY FOCUS AREAS

QbD APPROACH　•　HSM COLUMN SELECTION　•　GRADIENT OPTIMISATION　•　METHOD ROBUSTNESS