Practical scientific training for African laboratories.
© 2026 ChromAfrica LLCHPLC Training
Build confidence with every injection by moving step-by-step from method parameters to final chromatogram. Designed for analysts new to High-Performance Liquid Chromatography (HPLC), this practical course establishes fundamental routines for mobile phase preparation, system setup, sequence execution, and basic result interpretation.Participants will learn how to review standard HPLC-UV methods, configure instrumentation, execute analytical sequences, and troubleshoot common operational errors to prevent instrument downtime.Audience: Junior analysts, laboratory technicians, QA/QC assistants, and science studentsPrerequisites: No prior knowledge required – start from scratchTraining Format: Live online interactive session focused on practical setup and error-free operation
From method to chromatogram: build confidence with every injection.
AUDIENCE: JUNIOR ANALYSTS & LAB TECHNICIANS · LEVEL: BEGINNER · TRAINING TYPE: LIVE ONLINE INTERACTIVE SESSION
Operating an HPLC system efficiently requires mastering daily benchtop practices, understanding method parameters, and executing sequence runs without introducing common operational errors. Designed specifically for beginners, this course takes a step-by-step approach using a typical HPLC-UV method.
Participants walk through mobile phase preparation, instrument configuration, sample sequence loading, and basic chromatogram interpretation, establishing solid working practices that minimize downtime.
Establishing systematic routines for mobile phase degassing, purge cycles, and sequence setup eliminates 90% of routine HPLC operational issues.
Analysts and technicians new to HPLC or early in their analytical careers
Quality control and testing laboratory personnel requiring practical instrument operation skills
Science students and university graduates preparing for industry laboratory roles
Understand every parameter specified in a standard HPLC analytical method.
Prepare mobile phases, degas solvents, and set up fluidic lines correctly.
Configure HPLC hardware, pumps, injectors, column compartments, and UV detectors.
Create and execute sample injection sequences within instrument control software.
Interpret basic chromatographic results and resolve routine baseline or pressure issues.
Reviewing HPLC Method Parameters (Flow Rate, Isocratic/Gradient, Wavelength, Run Time)
Mobile Phase Preparation, Filtration, Degassing, and Solvent Compatibility
System Priming, Purging, and Stable Pressure Verification
Column Installation, Fitting Connections, and Equilibration Protocols
Sample and Standard Vials Preparation and Autosampler Loading
Setting Up Sequence Tables and Sample Injection Parameters
Monitoring Real-Time Chromatograms and Pressure Traces
Basic Peak Integration, Retention Time Check, and Calibration Curves
Identifying System Pressure Anomalies (High Pressure Drops, Pressure Spikes)
Resolving Air Bubbles in Pumps and Fluidic Lines
Basic Peak Anomaly Diagnostics (Peak Tailing, Split Peaks, Retention Drift)
Post-Run System Flushing, Column Washing, and Shutdown Procedures
Introduction to Biomolecule Characterization (Proteins, Peptides, mAbs, Oligonucleotides)
Size Exclusion Chromatography (SEC) for Aggregate and Purity Evaluation
Ion Exchange Chromatography (IEX) for Charge Variant Profiling
Reversed-Phase HPLC (RP-HPLC) for Peptide Mapping and Amino Acid Analysis
Hydrophilic Interaction (HILIC) and Hydrophobic Interaction (HIC) Overview
Includes step-by-step virtual HPLC-UV setup walkthroughs, mobile phase calculation exercises, sequence table creation tutorials, and real-time chromatogram evaluation scenarios.
Participants leave with a clear operational understanding of HPLC platforms, capable of preparing mobile phases, executing analytical sequences, interpreting chromatograms, and performing daily routine maintenance with confidence.
Practical scientific training for African laboratories.
© 2026 ChromAfrica LLC