From method to chromatogram: build confidence with every injection.
BEGINNER FRIENDLY
NO PRIOR KNOWLEDGE
HANDS‑ON FOCUS
- Designed for: anyone new to HPLC or early in their HPLC career
- No prior experience required – start from scratch
- Ideal for lab technicians, analysts, and students
- Step‑by‑step approach to running HPLC methods
- Focus on practical setup, sequence execution, and basic interpretation
- Build working practices that minimise common errors
Learning outcomes
- Understand each parameter in a typical HPLC method
- See how these parameters work in practice during a run
- Set up an HPLC system and method to minimise errors and maximise efficiency
- Perform sequences, interpret basic results, and troubleshoot common pitfalls
HPLC OPERATOR
LIVE ONLINE INTERACTIVE SESSION · step‑by‑step practical training
Using a typical HPLC-UV method, we work through reviewing the method, preparing mobile phase, setting up instrumentation, running sequences, and basic result interpretation. Focus on building working practices that avoid downtime.
🔬 INTRODUCTION TO BIOMOLECULE ANALYSIS
Overview of Biomolecules
- Importance of biomolecule characterization in research and industry
- Challenges in HPLC‑based biomolecule separation
Types of Biomolecules Analyzed by HPLC
- Amino Acids and Peptides
- Proteins and Their Structural Complexity
- Primary, secondary, tertiary, and quaternary structures
- Post‑Translational Modifications (PTMs)
- Phosphorylation, glycosylation, disulfide bonds
- Monoclonal Antibodies (mAbs)
- Antibody‑Drug Conjugates (ADC)
- Oligonucleotides and RNA‑Based Therapeutics
🧬 FUNDAMENTALS OF BIOTECHNOLOGY IN HPLC
Nucleotides and Nucleic Acids
- DNA, mRNA, and tRNA structures
- Transcription and translation processes
ICH Q6(B) Guidelines for Biopharmaceuticals
- Regulatory requirements for quality control and characterization
⚙️ HPLC TECHNIQUES FOR BIOMOLECULE ANALYSIS
Size Exclusion Chromatography (SEC) for Proteins
- Understanding Protein Aggregation and Its Impact
- SEC Column Selection and Mobile Phase Optimization
- Basic SEC Method Development for Biomolecule Purity Assessment
Ion Exchange Chromatography (IEX) for Charge Variants
- Charge Variability in Biomolecules
- Modes of IEX: Cation vs. Anion Exchange
- Optimizing pH and Salt Gradients for Selective Separation
Reversed‑Phase Chromatography (RPC) for Protein and Peptide Analysis
- Selection of Columns and Mobile Phases
- Separation of Proteins Based on Primary Structure
- Peptide Mapping and Characterization
- Derivatized Amino Acid Analysis Techniques
Hydrophilic Interaction Chromatography (HILIC) for Polar Biomolecules
- Overview of HILIC and Its Applications
- Glycan Analysis and Carbohydrate Profiling
- Un‑derivatized Amino Acid Analysis Using HILIC
Hydrophobic Interaction Chromatography (HIC) for Hydrophobic Proteins
- Fundamentals of HIC and Retention Mechanisms
- Common Applications in Protein and Antibody Analysis