A robust method survives transfer – validation proves it, optimization perfects it.
EXPERIENCED ANALYSTS
PRIOR HPLC KNOWLEDGE
METHOD TRANSFER FOCUS
- Designed for: those who want to understand common issues during HPLC method transfer
- Ideal for analysts, validation specialists, and lab managers
- Prerequisite: good prior understanding of HPLC
- Familiarity with method development is beneficial
Learning outcomes
- Overview of method validation and its role in method transfer
- Understand the HPLC method, robustness, and assessing parameter performance
- Master mobile phase chemistry: pH, pKa, additives, gradient elution
- Critical information about HPLC columns: physical parameters, chemical selectivity, protection
- Diagnose common problems related to mobile phase, column, sample prep, and instrument differences
METHOD VALIDATION AND OPTIMIZATION IN HPLC
LIVE ONLINE INTERACTIVE SESSION · method transfer & robustness
This training explores techniques for troubleshooting methods that show problems during transfer.
It builds a foundation in mobile phase design, column chemistry, and equipment differences, then applies that knowledge to a structured troubleshooting process.
📋 METHOD VALIDATION
- Overview of the validation process
- Reproducibility and method transfer
⚙️ THE HPLC METHOD
- Formal testing for method robustness
- Factor hazard and risk
- Method parameters
- Assessing factor criticality
💧 HPLC MOBILE PHASES
- pH, pKa – influence on retention and selectivity
- Mobile phase additives (buffers, ion‑pair reagents)
- Gradient elution principles and optimization
🧬 HPLC COLUMNS
- Physical parameters: length, ID, particle size, pore size
- Chemical selectivity – stationary phase chemistry (C18, phenyl, HILIC, etc.)
- Column protection: guard columns, filters, proper use
⚠️ COMMON PROBLEMS
- Issues relating to mobile phase (composition, pH, degassing)
- Issues relating to the column (contamination, aging, void)
- Issues relating to sample preparation (extraction, dilution, stability)
- Issues relating to differences in instrument design (dwell volume, mixing, detection)
💻 ONLINE PRACTICAL COMPONENT
- Case studies: method transfer failures and root cause analysis
- Interactive exercises on robustness testing and parameter criticality
ICH-compliant approach
Transfer strategies
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