Develop GC methods with confidence, not guesswork – every parameter tells a story.
EXPERIENCED GC USERS
PRIOR GC KNOWLEDGE
METHOD DEVELOPMENT
ADVANCED
- Designed for: experienced chromatographers with a good understanding of GC
- Attendees should have completed a basic GC course or have equivalent hands‑on experience
- Prerequisite: good knowledge of chromatography and experience as a GC user
- Some method development experience and a solid chemistry background are highly beneficial
Learning outcomes
- Set clear method development objectives aligned with analytical goals
- Optimize sample preparation, inlet conditions, and flow rates
- Choose the right column and design effective temperature programs
- Apply optimisation strategies – capacity factor, efficiency, selectivity, resolution
- Develop robust, validated GC methods with confidence
GC METHOD DEVELOPMENT
LIVE ONLINE INTERACTIVE SESSION · step‑by‑step from objectives to validation
This course provides a logical, progressive approach to GC method development. Through real‑world examples, tutorials, and exercises, you’ll learn to make the right decisions on column dimensions, stationary phases, inlet types, temperature programs, detector settings, and sample preparation – cutting development lead times drastically.
🎯 ESTABLISHING METHOD DEVELOPMENT OBJECTIVES
- Defining Analytical Goals
- Literature Search and Background Research
- Understanding What is Known vs. What Needs to Be Explored
- Introduction to Quality by Design (QbD)
- Identifying Critical Parameters and Robustness
🧪 SAMPLE PREPARATION CONSIDERATIONS
- Sample Clean‑Up Strategies
- Extraction Techniques
- Selecting Appropriate Solvents
⚙️ INLET, AND FLOW RATE OPTIMIZATION
- Effect of Split Ratio on Peak Shape and Quantification
- Investigating Oven Initial Temperature and Ramp Rates
- Conversion of Split Methods to Splitless Methods
- Optimizing Purge‑On Time
- Carrier Gas Selection and Flow Rate Optimization (van Deemter & Golay Theory)
🧬 COLUMN SELECTION AND TEMPERATURE PROGRAMMING
- Selecting the Right Stationary Phase
- Influence of Column Geometry on Separation
- Solute‑Stationary Phase Interactions
- Isothermal vs. Gradient Temperature Programming
- Theory and Practical Development of Temperature Gradients
📈 OPTIMIZATION STRATEGIES IN GC METHOD DEVELOPMENT
- Measuring and Optimizing:
- Capacity Factor
- Efficiency
- Resolution
- Selectivity
- Resolution Equation and Its Application
- Case Study: Developing an Effective GC Method
✅ FINALIZING AND VALIDATING THE METHOD
- Developing a GC Method for a Complex Mixture
- Systematic Instrument Setup and Validation
📊 DATA PROCESSING AND INTERPRETATION
- Peak Integration and Data Analysis
- Calibration Methods and Accuracy
- System Suitability Testing
🗣️ Q&A AND CLOSING DISCUSSION
- Summary of Key Concepts
- Open Discussion on Challenges Faced in GC Method Development
- Resources for Further Learning
💻 ONLINE PRACTICAL COMPONENT
- Interactive tutorials on inlet/column/detector choices
- Real‑world separation exercises and optimisation simulations
- Case‑based method development workshop