Detection Techniques for Mycotoxins and Toxigenic Fungi in the Food Chain

A five day seminar about fungi and mycotoxins in the food chain.

A five day seminar presented by
Dr. Sheila Okoth, University of Nairobi

To register for this seminar, contact Mr. John Angira by email or phone: +254-20-552096

Module 1
Overview on major toxigenic fungi and mycotoxins in plant disease.
Human and animal health effects and risk assessment of mycotoxins.
Mycotoxin analysis: an overview of various methodologies.
Taxonomy of toxigenic Aspergillus
Identification of Aspergillus toxigenic species occurring in maize.

Module 2
Analysis of fumonisins in maize / aflatoxins in groundnuts - sample preparation, extraction, cleanup, ELIZA determination.
Sampling methods for mycotoxin analysis.
Rapid methods for mycotoxin detection .
Prevention strategies and HACCP plans for mycotoxins in food safety.
Methods for isolation, identification and preservation of major toxigenic fungi.
Identification of Aspergillus toxigenic species occurring on cereals.
Analysis of fumonisins in maize / Aflatoxin in groundnuts - sample preparation, extraction, cleanup, ELIZA determination.

Module 3
Taxonomy of fumonisin-producing and related Fusarium species
Taxonomy of other toxin producing fungal species
Fusarium: species concepts and sexual fertility
Identification of Fusarium/Gibberella toxigenic species from cereals
Mycotoxin analysis in groundnuts - extraction, cleanup, HPLC determination
Identification of Fusarium/Gibberella toxigenic species from cereals
Mycotoxin analysis in groundnuts - extraction, cleanup, HPLC determination

Module 4
Overview of molecular methods for the identification of toxigenic Fusarium species
Molecular training in fungal identification: DNA extraction from fungal strain/food matrices, PCR, multiplex and Sequencing
Mycotoxin analysis in maize - extraction, cleanup, HPLC determination

Module 5
Mycotoxin legislation and performance criteria for analytical methods
Molecular training in fungal identification: DNA extraction from fungal strain/food matrices, PCR, multiplex and Sequencing
Mycotoxin analysis in maize - extraction, cleanup, HPLC determination
Questionnaire to participants for course evaluation and general discussion

COST = US $ 600
VENUE= Kenya Bureau of Standards, Nairobi

To register for this seminar, contact Mr. John Angira by email or phone: +254-20-552096

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