Modern HPLC
(Level 2 Training)

A workshop presented by Merlin K. L. Bicking, Ph.D. , of ACCTA, Inc.
Click here for scheduling information.

The trainees will benefit from the insights provided in this intensive three day course. Developed by chromatography experts, this course covers the essentials that every chromatographic needs to know, including:

• How to select columns and evaluate column performance
• How to avoid method transfer problems
• How to tell whether a symptom is caused by chemistry or hardware problems
• An unbiased evaluation of the new "UHPLC" technology
• How to set up a preventive maintenance schedule that will prevent problems from happening
• How to maximize column lifetime
• Why "equivalent" columns usually aren't
  

. . . and much, much more.

This course will provide time for students to ask questions and participate in discussions about HPLC. Students are encouraged to bring questions and examples from their own laboratories. Laboratory equipment will be available at some events, and demonstrations will be part of the discussion sessions. Each event will also offer time for vendor presentations and discussions with students.

• Day 1-Understanding Your HPLC System

  • Registration/Introductions
  • HPLC Basics
  • HPLC Components
    • Mobile Phase Reservoirs
    • Pumps and Mixing
    • Tubing and Fittings
    • Sample Injectors & Autosamplers
    • HPLC Detectors
  • Discussion
• Day 2-Advanced Operating Concepts for Your HPLC System
• Operating modes
  • Reversed Phase, Normal Phase, HILIC, etc.
• Operating Parameters
  • Column Configurations, Gradients, Column Selectivity, etc.
• Troubleshooting HPLC Systems
  • Symptoms and Solutions
• Sample Preparation for HPLC
• Discussion
• Day 3-Solving Problems With Your HPLC System
  • Integrating Chromatographic Peaks
  • Calibrating HPLC Systems
  • Recent Advances in HPLC
    • Fast LC, UPLC
  • Discussion
  • Laboratory Demonstrations (when available)
• Day 4-Vendor Presentations (when available)